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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
01/03/2019 |
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Actualizado : |
15/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
HAMOND, C.; SILVEIRA, C.S.; BURONI, F.; SUANES, A.; NIEVES, C.; SALABERRY, X.; ARÁOZ, V.; COSTA, R.A. DA; RIVERO, R.; GIANNITTI, F.; ZARANTONELLI, L. |
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Afiliación : |
CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENCIA BURONI, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; ALEJANDRA SUANES, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; CECILIA NIEVES, Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.//Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, UruguaY.; XIMENA SALABERRY, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ALMEIDA DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO RIVERO, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//6Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.; Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Montevideo, Uruguay./Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
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Título : |
Leptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Transboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133 |
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DOI : |
10.1111/tbed.13133 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019. |
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Contenido : |
Abstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH |
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Palabras claves : |
ACUTE LEPTOSPIROSIS; ENFERMEDADES DE LOS RUMIANTES; MOLECULAR DIAGNOSTICS; MORTALITY; PLATAFORMA DE SALUD ANIMAL; SALUD ANIMAL; SHEEP. |
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Thesagro : |
OVEJA; URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02629naa a2200373 a 4500
001 1059551
005 2020-01-15
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/tbed.13133$2DOI
100 1 $aHAMOND, C.
245 $aLeptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019.
520 $aAbstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH
650 $aOVEJA
650 $aURUGUAY
653 $aACUTE LEPTOSPIROSIS
653 $aENFERMEDADES DE LOS RUMIANTES
653 $aMOLECULAR DIAGNOSTICS
653 $aMORTALITY
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSALUD ANIMAL
653 $aSHEEP
700 1 $aSILVEIRA, C.S.
700 1 $aBURONI, F.
700 1 $aSUANES, A.
700 1 $aNIEVES, C.
700 1 $aSALABERRY, X.
700 1 $aARÁOZ, V.
700 1 $aCOSTA, R.A. DA
700 1 $aRIVERO, R.
700 1 $aGIANNITTI, F.
700 1 $aZARANTONELLI, L.
773 $tTransboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
29/07/2022 |
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Actualizado : |
02/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
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Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
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DOI : |
10.3389/fvets.2022.889157 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
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Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
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Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
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Marc : |
LEADER 03260naa a2200325 a 4500
001 1063470
005 2022-09-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fvets.2022.889157$2DOI
100 1 $aGIANNITTI, F.
245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022.
520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations.
653 $aABORTION
653 $aDAIRY CATTLE
653 $aGENETIC DIVERSITY
653 $aMolecular epidemiology
653 $aMultilocus microsatellite typin
653 $aNeospora caninum
653 $aPATHOLOGY
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aREPRODUCTIVE DISEASES
700 1 $aARÁOZ, V.
700 1 $aSILVEIRA, C.S.
700 1 $aFRANCIA, M.E.
700 1 $aROBELLO, C.
700 1 $aCABRERA, A.
773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
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